Determination of Oxalate in Urine Using Oxalate Oxidase: Comparison with Oxalate Decarboxylase
نویسندگان
چکیده
منابع مشابه
Determination of urinary oxalate with commercially available oxalate oxidase.
For this direct colorimetry of urinary oxalate, commercially available oxalate oxidase (EC 1.2.3.4) is used. The urine is first diluted, to diminish the effect of interfering substances. Analytical recovery of oxalate from urines with five different oxalate concentrations (0.4 to 2.0 mmol/L) ranged from 92 to 109% (mean 99%). The within-day and between-day precision (CV) of the method for a wid...
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1THE ACCURATE MEASUREMENT of oxalate concentration in urine presents considerable difficulties. The methods available in the literature are based upon either the direct formation of calcium oxalate precipitate and subsequent oxidimetric titration (1-3) or upon a preliminary ether extraction followed by a titrimetric procedure (4, .5). In a more elaborate method, the extraction with ether is com...
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We use oxalate oxidase from barley seedlings for the colorimetric determination of oxalate in plasma. The oxalate is converted to hydrogen peroxide, which, in the presence of peroxidase, is detected by a Trinder-like chromogenic system. Optimization of the assay, including deproteinization and elimination of interferences from reducing substrates, is described. Ascorbate additions (200 mumol/L)...
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We describe an enzymic colourimetric method for determination of oxalate level in urine using arylamine glass-bound sorghum leaf oxalate oxidase and horseradish peroxidase. The method is based on quantification of H2O2 generated from oxidation of urinary oxalate by immobilized oxalate oxidase, by a colour reaction consisting of 4-aminophnazone, phenol and immobilized peroxidase as chromogen. Mi...
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ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1979
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1979.17.5.309